Nov 24, 2004 03:08
19 yrs ago
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English term
Canada
Translations from start to Finnish
Native in: Finnish 
Works in: English to Finnish, French to Finnish, Dutch to Finnish, and 2 more.
1:10 volume
English
Medical
Chemistry; Chem Sci/Eng
"Add 1:10 volume Processing Buffer (0.5% sodium azide in 10X TBS) to the filtrate in order to bring the filtrate to 0.05% Azide in TBS."
Does that mean that I keep pouring the Processing Buffer into the filtrate until the volume of the cocktail is ten times the volume of the Processing Buffer that I pour, so that the concentration of the Azide, 0.5% in the buffer, drops to one tenth in the mix? Does "10X" in "10X TBS" have anything to do with that?
Does that mean that I keep pouring the Processing Buffer into the filtrate until the volume of the cocktail is ten times the volume of the Processing Buffer that I pour, so that the concentration of the Azide, 0.5% in the buffer, drops to one tenth in the mix? Does "10X" in "10X TBS" have anything to do with that?
Responses
| 5 +4 | Explanation |
Krisztina Vasarhelyi
|
Responses
+4
35 mins
Krisztina Vasarhelyi
Native in English
&
English
Medical and Scientific Translations
Native in: Hungarian 
, English
Works in: Hungarian to English, German to English
Related KudoZ points
:
4
Selected
Explanation
The Processing Buffer was prepared by mixing sodium azide with 10x TBS to get a final concentration of 0.5% sodium azide in the Buffer. The 10X refers only to the initial concentration of the TBS buffer used to make this solution.
Adding 1:10 volume of the processing buffer to the filtrate just means that you add one volume the processing buffer to 9 volumes of the filtrate which will result in a 1:10 dilution of the original 0.5% sodium azide.
For example, if you have 9 ml of the filtrate, you need to add 1 ml of the processing buffer, in order to produce a total volume of 10 mls. The diluted filtrate will then include a final concentration 0.05% sodium azide.
The 10X in the 10X TBS is also a way of expressing concentration. Going through the whole procedure described above, will also decrease the concentration of the TBS, but this is of no interest in the present context. The aim of the experiment is simply to achieve a final concentration of 0.05% sodium azide in the filtrate.
I hope this helps. Or is it even more confusing...?
Adding 1:10 volume of the processing buffer to the filtrate just means that you add one volume the processing buffer to 9 volumes of the filtrate which will result in a 1:10 dilution of the original 0.5% sodium azide.
For example, if you have 9 ml of the filtrate, you need to add 1 ml of the processing buffer, in order to produce a total volume of 10 mls. The diluted filtrate will then include a final concentration 0.05% sodium azide.
The 10X in the 10X TBS is also a way of expressing concentration. Going through the whole procedure described above, will also decrease the concentration of the TBS, but this is of no interest in the present context. The aim of the experiment is simply to achieve a final concentration of 0.05% sodium azide in the filtrate.
I hope this helps. Or is it even more confusing...?
4 KudoZ points awarded for this answer.
Comment: "No, that's fine."
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